- Jason Bourne as Adult Simba
- Marie Helena Kreutz as Adult Nala
- Alexander Conklin as Timon
- Ward Abbott as Pumbaa
- The Professor as Mufasa
- Nykwana Wombosi as Rafiki
- Danny Zorn as Zazu
- Nicolette Parsons as Young Simba
- Research Tech #1 as Banzai
- Research Tech #2 as Ed
- Giancarlo as Quint
- Eamon as Scar
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