- Wasabi-No-Ginger as Mufasa
- Cass as Sarabi
- GoGo Tomago as Sarafina
- Hiro Hamada as Young Simba
- Margo (from Despicable Me) as Young Nala
- Tadashi as Adult Simba
- Honey Lemon as Adult Nala
- Fred as Zazu
- Flint Lockwood (from Cloudy with a Chance of Meatballs) as Timon
- Baymax as Pumbaa
- Dr. Nefario (from Despicable Me) as Rafiki
- Robert Callaghan as Scar
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